Proteomika: Béda antara owahan

[[Gambar:proteinprotéin pattern analyzer.jpg|300px|thumb|right|Matrix-assisted laser desorption/ionization (MALDI), piranti yang digunakan untuk mempelajari proteinprotéin.]]

'''Protèomika''' punika kajian kanthi molékular kaliyan sédaya [[proteinprotéin]] ingkang dipunhasilakén saking [[èksprèsi genetik|èksprèsi gen]] ing [[sèl (biologi)|sèl]], mliginipun ngenai struktur saha mupangatipun.<ref name="pmid9740045">{{cite journal |author=Anderson NL, Anderson NG |title=Proteome and proteomics: new technologies, new concepts, and new words |journal=Electrophoresis |volume=19 |issue=11 |pages=1853–61 |year=1998 |pmid=9740045 |doi=10.1002/elps.1150191103}}</ref><ref name="pmid10189717">{{cite journal |author=Blackstock WP, Weir MP |title=Proteomics: quantitative and physical mapping of cellular proteins |journal=Trends Biotechnol. |volume=17 |issue=3 |pages=121–7 |year=1999 |pmid=10189717| doi = 10.1016/S0167-7799(98)01245-1}}</ref> sadaya proteinprotéin ing salebeting sèl dados ''proteom''.<ref name="wilkins1996" /> Istilah ''proteomik'' ingkang wiwit dipuntepang ing taun 1997,ugi dipundamel adhedhasar analogi [[genetika]] kanggé ilmi kanggé pasianaon babagan [[gen]].<ref>{{cite journal |author=P. James |title=Protein identification in the post-genome era: the rapid rise of proteomics.|journal=Quarterly reviews of biophysics|volume=30 |issue=4|pages=279–331 |year=1997 |pmid=9634650 |doi=10.1017/S0033583597003399}}</ref> kanggé istilah ''proteom'' piyambak, saking gabungan istilah proteinprotéin lan [[genom]] ingkang dipunkemukaaken déning [[Marc Wilkins]] ing taun [[1994]] ing wekdal, mendet gelar PhD.<ref name="wilkins1996">{{cite journal |author=Marc R. Wilkins, Christian Pasquali, Ron D. Appel, Keli Ou, Olivier Golaz, Jean-Charles Sanchez, Jun X. Yan, Andrew. A. Gooley, Graham Hughes, Ian Humphery-Smith, Keith L. Williams & Denis F. Hochstrasser|title=From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis|journal=Nature Biotechnology |volume=14 |issue=1 |pages=61–65 |year=1996 |pmid=9636313 |doi=10.1038/nbt0196-61|accessdate=2009-01-15}}</ref><ref>[http://www.babs.unsw.edu.au/directory.php?personnelID=12 UNSW Staff Bio: Professor Marc Wilkins]</ref> Salah satu piranti yang umumnya digunakan untuk ngèlmu ini ya iku ''matrix-assisted laser desorption/ionization'' (MALDI).<ref name="Klopfleisch1">{{cite journal | author =Klopfleisch R, Gruber AD.| title = Increased expression of BRCA2 and RAD51 in lymph node metastases of canine mammary adenocarcinomas.| journal = Veterinary Pathology | volume = 46 | issue = 3 | pages =416–22 | year = 2009 | pmid = 19176491| doi=10.1354/vp.08-VP-0212-K-FL}}</ref>

Pinten-pinten jinis métodhe sampun dirembaake kanggé pasinaon [[proteinprotéin]].<ref name="brock">{{cite book

}}</ref> dipunwiwiti ing abad 1, proteomika ngginakaken analisis 2D arupa gel elektroforesis poliakrilamida.<ref name="brock"/> kaliyan ngginakaken tèhnik punika, proteinprotéin ing sampel saged dipunkapisahaken, diindentifikasiaken ,saha dipunukur adhedhasar abot molekulipun.<ref name="brock"/> kaliyan ngginakaken analisis punika, pinten-pinten jinis proteinprotéin ingkang dipunkasilaken déning baktèri, kados ta ''[[Escherichia coli]]'',sampun kasil dipunpisahaken saha dipunpurifikasiaken.<ref name="brock"/> Teknologi sanès dipunrembaaken [[spektrometri massa]] ingkang asifat sensitif.<ref name="brock"/> Di samping itu, [[Kromatografi cair berperforma tinggi]] (HPLC) ugi saged dipun-ginakaken ing sampel diginaake ing kolom tekanan inggil saha proteinprotéin ingkang ngandut ing kaiket kaliyan [[matriks]] ingkang wonten.<ref name="brock"/>

Proteomika, merdamel sami kaliyan Salvat Biotech, sampun ngidentifikasi sebuah panèl biomarker urin kanggé diagnosis kekambuhan kanker kandung kemih ing pasien ingkang sadèrèngipun dipunrawat amergi kahanan lan sampun ngembangaken test kit immunoassay kanggé penggunaan in vitro diagnostik. Produk punika kapérang sakingi array solusi berbasis manik ingkang kanthi bersamaan mengkuantifikasi penanda pinten-pinten proteinprotéin ing urin pasien lan saged dipun-ginakaken kanggé memperkirakan risiko kekambuhan lelara.